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England defenders Kyle Walker and Harry Maguire, and back-up goalkeeper Sam Johnstone, have been ruled out of Tuesday’s friendly with Belgium.
Burnley keeper James Trafford and Manchester City defender Rico Lewis, who were with the England Under-21s, have been promoted to the senior squad.
Trafford, 21, helped England win the Euro Under-21 last summer and this is his first senior call-up.
Lewis, 19, made his Three Lions debut in November against North Macedonia.
His City team-mate Walker was injured in the first half of Saturday’s 1-0 friendly defeat by Brazil at Wembley.
Manchester United’s Maguire, who replaced Walker as captain when he was substituted, came off in the 67th minute.
Maguire, Walker and Crystal Palace’s Johnstone, who did not feature on Saturday, have returned to their clubs.
Bayern Munich striker Harry Kane and Arsenal winger Bukayo Saka have already been ruled out.